|
KMID : 0665220080210030263
|
|
Korean Journal of Food and Nutrition
2008 Volume.21 No. 3 p.263 ~ p.268
|
|
|
Effects of Codonopsis lanceolata Extracts on Mouse Immune Cell Activation
|
|
Ryu Hye-Sook
|
|
|
Abstract
|
|
|
|
Codonopsis lanceolata has long been used as a seasonal food and as a traditional tonic medicine with anti-inflammatory and anti-oxidation properties. The present study investigated the in vitro effect of Codonopsis lanceolata extracts on immune function in mice. After preparing a single cell suspension splenocyte proliferation was determined by the MTT(3-[4,5-dimethylthiazol-2-y]-2,5-diphenyl terazolium bromide) assay. The cytokines IL-1, IL-6, and TNF- were not secreted by macrophages stimulated with or without LPS as determined by an ELISA cytokine kit assay. After a 48-hr incubation with the mitogens ConA or LPS there was an increase in splenocytes proliferation and in the production of IL-1, IL-6, and TNF- in the suspensions supplemented with 50, 100, 250, 500 Codonopsis lanceolata water extract. The results suggest Codonopsis lanceolata water extract may enhance immune function by regulating splenocyte proliferation and stimulating cytokine production.
|
|
|
KEYWORD
|
|
|
IL-1β, IL-6, TNF-α, Codonopsis lanceolata, splenocyte proliferation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|